Shallow and deep-water benthic samples collected during two cruises to northern Cyprus held in 1997 and 1998 showed relatively high species richness of Eusyllinae and Syllinae, with a total of 49 species. The materials contained one species (
Eusyllis kupfferi Langerhans, 1879) new to the Mediterranean Sea, seven species new to the Eastern Mediterranean Sea, 23 species new to the Levantine Sea and 42 species new to Cyprus. The morphological, biometrical and distributional characteristics of the species as well as a checklist of the Eusyllinae and Syllinae species reported from the Levant coast are provided.